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Recombinant Rabbit Monoclonal Antibodies > PUMA Rabbit mAb
PUMA Rabbit mAb#56073
Yes
All lanes : BBC3 Rabbit mAb at 1/1k dilution
Lane 1 : HL60 whole cell lysates
Lane 2 : K562 whole cell lysates Lane 3 : PC3 whole cell lysates Lane 4 : HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 8 seconds
All lanes : BBC3 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse Liver lysates
Lane 2 : Mouse Kidney lysates Lane 3 : Rat Liver lysates Lane 4 : Rat Kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 8 seconds
Formalin-fixed, paraffin-embedded human breast carcinom tissue stained for BBC3 using 56073 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse lung tissue stained for BBC3 using 56073 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat lung tissue stained for BBC3 using 56073 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - BBC3 antibody (56073)
56073 staining BBC3 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56073 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control, purified 56073 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
