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Location： Home > KD/KO Validation > p62/SQSTM1 Rabbit mAb

p62/SQSTM1 Rabbit mAb#56089

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Product Detail

Product Namep62/SQSTM1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman Mouse Rat

Specificityp62/SQSTM1 Antibody detects endogenous levels of total p62/SQSTM1

Immunogen DescA synthesized peptide derived from human p62/SQSTM1

ConjugateUnconjugated

Other NamesOSIL; Oxidative stress induced like; p60; p62; p62B; Paget disease of bone 3;PDB 3; PDB3;

Accession NoUniprot:Q13501

Uniprot Q13501

Calculated MW48 kDa

Sdspage MW62 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Western blot analysis of p62/SQSTM1 expression in SKBR-3 cell lysate.
Immunofluorescent analysis of Hela cells, using p62/SQSTM1 Antibody.
All lanes : p62/SQSTM1 Rabbit mAb at 1/1k dilution

Lane 1 : A549 cell lysate
Lane 2 : JK cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48kDa
Observed band size: 62kDa
All lanes : p62/SQSTM1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : p62/SQSTM1 knockout HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded Human colon carcinoma tissue stained for p62/SQSTM1 using 56089 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 56089, please notify us so that we can cite your literature.

et al,MAPK14/AIFM2 pathway regulates mitophagy-dependent apoptosis to improve atrial fibrillation , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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