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Cyclin A1/A2 Rabbit mAb#56107

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Product Detail

Product NameCyclin A1/A2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC IP

Species ReactivityHuman

SpecificityCyclin A1/A2 Antibody detects endogenous levels of Cyclin A1/A2

Immunogen DescA synthesized peptide derived from human Cyclin A1/A2

ConjugateUnconjugated

Other NamesCCN1; CCNA; CCNA1; CCNA2; CT146; Cyclin-A; Cyclin-A1; Cyclin-A2;

Accession NoUniprot:P20248/P78396

Uniprot P20248/P78396

Calculated MW52,49kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:1000
IHC:1:100~1:500
IP:1:50

All lanes: Cyclin A1/A2 Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 49,52 kDa
Observed band size: 49,52 kDa

Exposure time: 3 seconds
All lanes:Cyclin A1/A2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : Cyclin A1/A2 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for Cyclin A1/A2 using 56017 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 56107, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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