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CD276 Rabbit mAb#56177

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Product Detail

Product NameCD276 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IP FC

Species ReactivityHuman Mouse

SpecificityCD276 Antibody detects endogenous levels of total CD276

Immunogen DescA synthesized peptide derived from human CD276

ConjugateUnconjugated

Other Names4Ig B7 H3; B7 homolog 3; B7H3; B7RP-2; CD276 antigen; CD276 molecule;

Accession NoUniprot:Q5ZPR3

Uniprot Q5ZPR3

Calculated MW100kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IP:1:50
FC:1:200

All lanes: CD276 Rabbit mAb at 1/1k dilution

Lane 1 : 293T whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 100 kDa

Exposure time: 6 seconds
All lanes :CD276 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CD276 knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.

If you have published an article using product 56177, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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