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CD82 Rabbit mAb#56256

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Product Detail

Product NameCD82 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC FC

Species ReactivityHuman Rat

SpecificityCD82 Antibody detects endogenous levels of total CD82

Immunogen DescA synthesized peptide derived from human CD82

ConjugateUnconjugated

Other NamesC33; CD82; IA4; Inducible membrane protein; KAI1; Kangai1; SAR2; ST6; Tetraspanin 27; Tspan27;

Accession NoUniprot:P27701

Uniprot P27701

Calculated MW30-60kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IHC:1:50~1:200
FC:1:50

All lanes: CD82 Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 30-60 kDa

Exposure time: 7 seconds
All lanes:CD82 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 : CD82 knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.

If you have published an article using product 56256, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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