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ABHD5 Rabbit mAb#56401

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Product Detail

Product NameABHD5 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC FC

Species ReactivityHuman

SpecificityABHD5 Antibody detects endogenous levels of total ABHD5

Immunogen DescA synthesized peptide derived from human ABHD5

ConjugateUnconjugated

Other NamesABHD5; Abhydrolase domain containing 5; CDS; CGI58; IECN2; NCIE2;

Accession NoUniprot:Q8WTS1

Uniprot Q8WTS1

Calculated MW45kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IHC:1:100~1:500
FC:1:100

All lanes: ABHD5 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : MCF7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 39 kDa
Observed band size: 45 kDa

Exposure time: 3 seconds
All lanes:ABHD5 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : ABHD5 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for ABHD5 using 56401 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 56401, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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