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CD133 Rabbit mAb#56954

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Product Detail

Product NameCD133 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman

SpecificityCD133 Antibody detects endogenous levels of total CD133

Immunogen DescA synthesized peptide derived from human CD133

ConjugateUnconjugated

Other NamesAC133; CD133; CORD12; hProminin; MCDR2; PROM1; Prominin like 1; Prominin1; PROML1; RP41; STGD4

Accession NoUniprot:O43490

Uniprot O43490

Calculated MW97 kDa

Sdspage MW133 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:2000
IHC:1:50~1:200
Western blot analysis of CD133 expression in HT-29 cell lysate.
All lanes : CD133 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CD133 knockout HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded Human bladder carcinoma tissue stained for CD133 using 56954 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 56954, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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