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Location： Home > Phospho Antibodies > Recombinant Tau(Phospho-Thr181) Rabbit mAb(G69)

Recombinant Tau(Phospho-Thr181) Rabbit mAb(G69)#58002

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Product NameRecombinant Tau(Phospho-Thr181) Rabbit mAb(G69)

Clone No.G69

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationAffinity purification

ApplicationsELISA,WB,IHC,IF

Species ReactivityHu, Ms, Rt

SpecificityThe antibody detects endogenous level of Tau only when phosphorylated at threonine 181.

Immunogen TypePeptide

Immunogen DescPeptide sequence around phosphorylation site of threonine 181 derived from Human Tau.

Target NameTau

ConjugateUnconjugated

Other NamesMAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau

Accession NoSwiss-Prot: P10636
NCBI Protein: NP _001116538.1

Uniprot P10636

Calculated MWPredicted MW: 48 62 78 kd

Sdspage MW79/50-80 kDa

Concentration0.8 mg/ml

FormulationPBS with 0.02% sodium azide,pH7.3.

StorageUpon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.

Application Details
Elisa:1:10000
WB: 1:500~1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Western blot analysis of extracts from 293 cells, transfected with Tau and treated with calf intestinal phosphatase (CIP) using Tau(Phospho-Thr181) Rabbit mAb.
All lanes : Recombinant Tau(Phospho-Thr181) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Tau(Phospho-Thr181) knockout HAP1 cell lysate
Lane 3 : Mouse Brain lysates
Lane 4 : Rat Brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 79 kDa
Observed band size: 50-80 kDa

Exposure time: 7 seconds Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded Mouse brain tissue stained for Tau(Phospho-Thr181) using 58002 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Tau(Phospho-Thr181) antibody (58002)

ICC/IF staining of Tau(Phospho-Thr181) in mouse brain tissue. mouse brain was fixed with 4% Paraformaldehyde permeabilized for 20 minutes.
Samples were incubated with 58002 at a working dilution of 1/50. The secondary antibody was Alexa Fluor 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI. Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Tau(Phospho-Thr181) antibody (58002)

ICC/IF staining of Tau(Phospho-Thr181) in mouse brain tissue. mouse brain was fixed with 4% Paraformaldehyde permeabilized for 20 minutes.
Samples were incubated with 58002 at a working dilution of 1/50. The secondary antibody was Alexa Fluor 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

 Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. 

Puig B, et al.(2005) Acta Neuropathol (Berl). 110(3):261-268. 
 

If you have published an article using product 58002, please notify us so that we can cite your literature.

et al,Fusobacterium nucleatum infection-induced neurodegeneration and abnormal gut microbiota composition in Alzheimer's disease-like rats , (2022),
PMID: 36188448

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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