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NF-κB (p105/p50) Rabbit mAb#58548

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Product NameNF-κB (p105/p50) Rabbit mAb

Clone No.AB9

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityNF-κB (p105/p50) Antibody detects endogenous levels of total NF-κB (p105/p50)

Immunogen DescA synthesized peptide derived from human NF-κB (p105/p50)

Target NameNFKB1

Other NamesNF-kappa-B1; p84/NF-kappa-B1 p98; NFKB1; DNA-binding factor KBF1; EBP-1; NFkB-p50;

Accession NoUniprot:P19838

Uniprot P19838

Calculated MWPredicted band size: 50,120 kDa

Sdspage MW50,120 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C in dark for 6 months.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: NF-κB (p105/p50) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50,120 kDa
Observed band size: 50,120 kDa

Exposure time: 8 seconds
All lanes:NF-κB (p105/p50) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HCT116 cell lysate
Lane 2 : NF-κB (p105/p50) knockdown HCT116 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for NF-κB (p105/p50) using 58548 at 1/100 dilution in immunohistochemical analysis.

NFkB-p105 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.

If you have published an article using product 58548, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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