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Caspase-9 Rabbit mAb#58555

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Product Detail

Product NameCaspase-9 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF FC IP

Species ReactivityHuman

SpecificityCaspase-9 Antibody detects endogenous levels of total Caspase-9

Immunogen DescA synthesized peptide derived from human Caspase-9

Other NamesAPAF-3; Caspase9; ICE-LAP6; Apoptotic protease Mch-6; CASP-9; Caspase-9; Caspase-9 precursor; MCH6; RNCASP9;

Accession NoUniprot:P55211

Uniprot P55211

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
Western blot analysis of Caspase-9 in HeLa cell lysate treated with Camptothecin.
Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody.
Immunofluorescent analysis of 3T3 cells, using Caspase-9 Antibody.
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.

If you have published an article using product 58555, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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