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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Vinculin Rabbit mAb

Vinculin Rabbit mAb#58623

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Product Detail

Product NameVinculin Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF IP

Species ReactivityHuman Mouse Rat

SpecificityVinculin Antibody detects endogenous levels of total Vinculin

Immunogen DescA synthesized peptide derived from human Vinculin

Other NamesVinculin;VCL;Metavinculin; MV;

Accession NoUniprot:P18206

Uniprot P18206

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:10000~1:20000 ICC/IF 1:50~1:200 IP 1:50
Western blot analysis of Vinculin expression in (1) HepG2 cell lysate; (2) HeLa cell lysate.
Immunofluorescent analysis of A673 cells, using Vinculin Antibody .
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail. In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration.

If you have published an article using product 58623, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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