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Recombinant Rabbit Monoclonal Antibodies > STAT1 Rabbit mAb
STAT1 Rabbit mAb#58661
Yes
All lanes: STAT1 Rabbit mAb at 1/1k dilution
Lane 1 : 293 whole cell lysates Lane 2 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 84 kDa
Exposure time: 3 seconds
All lanes: STAT1 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse lung lysates Lane 2 : Mouse kidney lysates Lane 3 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 84 kDa
Exposure time: 5 seconds
All lanes :STAT1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STAT1 knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for STAT1 using 58661 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse colon tissue stained for STAT1 using 58661 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence STAT1 antibody (58661) ICC/IF staining of STAT1 in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 58661 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
