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Ubiquitin Rabbit mAb#58835

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Product Detail

Product NameUbiquitin Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF FC

Species ReactivityHuman Mouse Rat

SpecificityUbiquitin Antibody detects endogenous levels of total Ubiquitin

Immunogen DescA synthesized peptide derived from human Ubiquitin

Other NamesFLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

Accession NoUniprot:P0CG47

Uniprot P0CG47

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
Western blot analysis of Ubiquitin expression in 293T cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon, using Ubiquitin Antibody.
Immunofluorescent analysis of Raji cells, using Ubiquitin Antibody .
Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

If you have published an article using product 58835, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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