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Location： Home > Recombinant Rabbit Monoclonal Antibodies > 5T4 Rabbit mAb

5T4 Rabbit mAb#58900

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Product Detail

Product Name5T4 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman

Specificity5T4 Antibody detects endogenous levels of 5T4

Immunogen DescA synthesized peptide derived from human 5T4

Other NamesTPBG; 5T4; 5T4-AG; 5T4AG; 5T4 oncofetal antigen; M6P1; Trophoblast glycoprotein;

Accession NoUniprot:Q13641

Uniprot Q13641

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:500~1:1000 IHC 1:50~1:200
Western blot analysis of 5T4 expression in Caco-2 cell lysates.
Immunohistochemical analysis of paraffin-embedded human uters cancer, using 5T4 Antibody.
5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1�C60.

If you have published an article using product 58900, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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