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Insulin Receptor Rabbit mAb#59060

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Product Detail

Product NameInsulin Receptor Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityInsulin Receptor Antibody detects endogenous levels of Insulin Receptor

Immunogen DescA synthesized peptide derived from human Insulin Receptor

Other NamesCD220; HHF5; human insulin receptor; Insr; Insulin receptor subunit beta; IR 1; IR;

Accession NoUniprot:P06213

Uniprot P06213

Calculated MWPredicted band size: 156 kDa

Sdspage MWObserved band size: 95 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Insulin Receptor Rabbit mAb at 1/1k dilution

Lane 1 : HepG2 whole cell lysates
Lane 2 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 156 kDa
Observed band size: 95 kDa

Exposure time: 4 seconds
All lanes: Insulin Receptor Rabbit mAb at 1/1k dilution

Lane 1 : Mouse liver lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat liver lysates
Lane 4 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 156 kDa
Observed band size: 95 kDa

Exposure time: 6 seconds
All lanes:Insulin Receptor Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HCT116 cell lysate
Lane 2 :Insulin Receptor knockdown HCT116 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Insulin Receptor using 59060 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Insulin Receptor antibody (59060)

ICC/IF staining of Insulin Receptor in BxPC-3 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59060 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Insulin Receptor is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues; Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin.

If you have published an article using product 59060, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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