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Rad50 Rabbit mAb#59502

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Product Detail

Product NameRad50 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityRad50 Antibody detects endogenous levels of total Rad50

Immunogen DescA synthesized peptide derived from human Rad50

Other NameshRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 PEN; RAD502; Rad50l;

Accession NoUniprot:Q92878

Uniprot Q92878

Calculated MWPredicted band size: 154 kDa

Sdspage MWObserved band size: 154 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Rad50 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : Mouse lung lysates
Lane 4 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 154 kDa
Observed band size: 154 kDa

Exposure time: 7 seconds
All lanes:Rad50 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :Rad50 Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Rad50 using 59502 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Rad50 antibody (59502)

ICC/IF staining of Rad50 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59502 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A.

If you have published an article using product 59502, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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