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GLUR Rabbit mAb#59516

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Product Detail

Product NameGLUR Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityGLUR Antibody detects endogenous levels of total GLUR

Immunogen DescA synthesized peptide derived from human GLUR

Other NamesGLR1; GLUR; Glutathione reductase; Glutathione reductase mitochondrial; Glutathione reductase, mitochondrial; GR; Gr1; GRase; GRD1; GSR;

Accession NoUniprot:P00390

Uniprot P00390

Calculated MWPredicted band size: 56 kDa

Sdspage MWObserved band size: 56 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: GLUR Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat kidney lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 7 seconds
All lanes:GLUR Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HCT116 cell lysate
Lane 2 :GLUR Rabbit mAb knockdown HCT116 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver tissue stained for GLUR using 59516 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence GLUR antibody (59516)

ICC/IF staining of GLUR in A549 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59516 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Maintains high levels of reduced glutathione in the cytosol.

If you have published an article using product 59516, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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