Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Recombinant Rabbit Monoclonal Antibodies > Dynamin 2 Rabbit mAb

Dynamin 2 Rabbit mAb#59532

Availability: Yes
Total Amount:$
Product Detail

Product NameDynamin 2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityDynamin 2 Antibody detects endogenous levels of total Dynamin 2

Immunogen DescA synthesized peptide derived from human Dynamin 2

Other NamesCMT2M; CMTDI1; CMTDIB; DI CMTB; Dnm2; DYN II; DYN2; Dynamin II; Dynamin2; DynaminII; DYNII;

Accession NoUniprot:P50570

Uniprot P50570

Calculated MWPredicted band size: 98 kDa

Sdspage MWObserved band size: 98 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Dynamin 2 Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lane 3 : PC12 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 98 kDa
Observed band size: 98 kDa

Exposure time: 4 seconds
All lanes:Dynamin 2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :Dynamin 2 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung tissue stained for Dynamin 2 using 59532 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Dynamin 2 antibody (59532)

ICC/IF staining of Dynamin 2 in PC-12 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59532 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.

If you have published an article using product 59532, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;