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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MCM7 Rabbit mAb

MCM7 Rabbit mAb#59558

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Product Detail

Product NameMCM7 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityMCM7 Antibody detects endogenous levels of total MCM7

Immunogen DescA synthesized peptide derived from human MCM7

Other NamesCDC47 homolog; P1.1-MCM3; MCM7; CDC47; MCM2; P1CDC47; P85MCM; PNAS146; PPP1R104;

Accession NoUniprot:P33993

Uniprot P33993

Calculated MWPredicted band size: 81 kDa

Sdspage MWObserved band size: 81 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: MCM7 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 81 kDa
Observed band size: 81 kDa

Exposure time: 9 seconds
All lanes:MCM7 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :MCM7 Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for MCM7 using 59558 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence MCM7 antibody (59558)

ICC/IF staining of MCM7 in SH-SY5Y cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59558 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.

If you have published an article using product 59558, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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