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KAT7 / HBO1 / MYST2 Rabbit mAb#59576

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Product Detail

Product NameKAT7 / HBO1 / MYST2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityKAT7 / HBO1 / MYST2 Antibody detects endogenous levels of total KAT7 / HBO1 / MYST2

Immunogen DescA synthesized peptide derived from human KAT7 / HBO1 / MYST2

Other NamesHBO1; HBOa; KAT7; MOZ; MYST 2; MYST protein 2; MYST2; SAS 2; SAS2 and TIP60 protein 2; TIP60 protein 2; YBF2/SAS3; ZC2HC7;

Accession NoUniprot:O95251

Uniprot O95251

Calculated MWPredicted band size: 71 kDa

Sdspage MWObserved band size: 75 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: KAT7 / HBO1 / MYST2 Rabbit mAb at 1/1k dilution

Lane 1 : MCF-7 whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lane 3 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 71 kDa
Observed band size: 75 kDa

Exposure time: 4 seconds
All lanes:KAT7 / HBO1 / MYST2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :KAT7 / HBO1 / MYST2 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for KAT7 / HBO1 / MYST2 using 59576 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence KAT7 / HBO1 / MYST2 antibody (59576)

ICC/IF staining of KAT7 / HBO1 / MYST2 in HUVEC cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59576 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.

If you have published an article using product 59576, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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