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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Ephrin B2 Rabbit mAb

Ephrin B2 Rabbit mAb #59580

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Product Detail

Product NameEphrin B2 Rabbit mAb

Clone No.31D11

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityEphrin B2 Antibody detects endogenous levels of total Ephrin B2

Immunogen DescA synthesized peptide derived from human Ephrin B2

Target NameEFNB2

Other NamesEfnb2; ephrin B2; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK5;

Accession NoUniprot:P52799

Uniprot P52799

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4℃ short term. Store at -20℃ long term. Avoid freeze / thaw cycle.

Application Details

WB 1:500~1:2000 ICC/IF 1:50~1:200

All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1:HeLa.Lane2:293.Lane3:MCF-7.Lane4:K562.Lane5:U-87 MG.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1: HEK293 cell lysate.Lane2:Mouse spleen lysate.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1:K562.Proteins at 20ug per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1:Mouse kidney.Lane2:Mouse brain.Lane3:Rat kidney.Lane4:Rat brain.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
Immunofluorescent analysis using the Antibody at 1:50 dilution.4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells.

If you have published an article using product 59580, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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