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NFkB p100 / p52 Rabbit mAb #59594

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Product Detail

Product NameNFkB p100 / p52 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF IP

Species ReactivityHuman Mouse Rat

SpecificityNFkB p100 / p52 Antibody detects endogenous levels of total NFkB p100 / p52

Immunogen DescA synthesized peptide derived from human NFkB p100 / p52

Other NamesCVID10; DNA binding factor KBF2; H2TF1; Lyt10; NFKB2; Nuclear factor NF kappa B p100 subunit; Nuclear factor NF kappa B p52 subunit; Oncogene Lyt 10; p105; p49/p100;

Accession NoUniprot:Q00653

Uniprot Q00653

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:1000~1:5000 ICC/IF 1:50~1:200 IP 1:50
Western blot analysis of NFkB p100 / p52 expression in (1) Jurkat cell lysate; (2) Mouse heart lysate.
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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