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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MAPKAP Kinase 2 Rabbit mAb

MAPKAP Kinase 2 Rabbit mAb#59659

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Product Detail

Product NameMAPKAP Kinase 2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityMAPKAP Kinase 2 Antibody detects endogenous levels of total MAPKAP Kinase 2

Immunogen DescA synthesized peptide derived from human MAPKAP Kinase 2

Other NamesMAPKAP K2; MAPKAP kinase 2; MAPKAPK 2; MAPKAPK2; Mitogen activated protein kinase activated protein kinase 2; MK 2;

Accession NoUniprot:P49137

Uniprot P49137

Calculated MWPredicted band size: 46 kDa

Sdspage MWObserved band size: 46 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes: MAPKAP Kinase 2 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 5 seconds
All lanes:MAPKAP Kinase 2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HCT116 cell lysate
Lane 2 :MAPKAP Kinase 2 Rabbit mAb knockdown HCT116 cell lysate

Lysates/proteins at 20 µg per lane.
Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity).

If you have published an article using product 59659, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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