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DCP1A Rabbit mAb#59727

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Product Detail

Product NameDCP1A Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityDCP1A Antibody detects endogenous levels of total DCP1A

Immunogen DescA synthesized peptide derived from human DCP1A

Other NamesDcp1a; HSA275986; Nbla00360; SMAD4IP1; SMIF;

Accession NoUniprot:Q9NPI6

Uniprot Q9NPI6

Calculated MWPredicted band size: 63 kDa

Sdspage MWObserved band size: 75 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: DCP1A Rabbit mAb at 1/1k dilution

Lane 1 : 293T whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 63 kDa
Observed band size: 75 kDa

Exposure time: 6 seconds
All lanes:DCP1A Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :DCP1A Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for DCP1A using 59727 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence DCP1A antibody (59727)

ICC/IF staining of DCP1A in HepG2 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59727 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.

If you have published an article using product 59727, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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