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Casein Kinase 2 beta Rabbit mAb#59773

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Product Detail

Product NameCasein Kinase 2 beta Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityCasein Kinase 2 beta Antibody detects endogenous levels of total Casein Kinase 2 beta

Immunogen DescA synthesized peptide derived from human Casein Kinase 2 beta

Other NamesCK II beta; CK2B; CK2N; CSK2B; CSNK 2B; csnk2b; G5A; Phosvitin; Protein G5a;

Accession NoUniprot:P67870

Uniprot P67870

Calculated MWPredicted band size: 25 kDa

Sdspage MWObserved band size: 25 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Casein Kinase 2 beta Rabbit mAb at 1/1k dilution

Lane 1 : HepG2 whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lane 3 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Exposure time: 7 seconds
All lanes:Casein Kinase 2 beta Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :Casein Kinase 2 beta Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Casein Kinase 2 beta using 59773 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Casein Kinase 2 beta antibody (59773)

ICC/IF staining of Casein Kinase 2 beta in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59773 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.

If you have published an article using product 59773, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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