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PCAF Rabbit mAb#59776

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Product Detail

Product NamePCAF Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityKAT2B / PCAF Antibody detects endogenous levels of total KAT2B / PCAF

Immunogen DescA synthesized peptide derived from human KAT2B / PCAF

Other NamesCAF; GCN5; GCN5L; GCN5L1; KAT2B; P/CAF; p300/CBP��P300/CBP-associated factor��Lysine acetyltransferase 2B��PCAF

Accession NoUniprot:Q92831

Uniprot Q92831

Calculated MWPredicted band size: 93 kDa

Sdspage MWObserved band size: 93 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes: PCAF Rabbit mAb at 1/1k dilution

Lane 1 : A431 whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 93 kDa
Observed band size: 93 kDa

Exposure time: 4 seconds
All lanes:PCAF Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :PCAF Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A.

If you have published an article using product 59776, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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