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STUB1 Rabbit mAb#59818

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Product Detail

Product NameSTUB1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificitySTUB1 Antibody detects endogenous levels of total STUB1

Immunogen DescA synthesized peptide derived from human STUB1

Other NamesSTUB1; CHIP; HSPABP2; NY-CO-7; SDCCAG7; UBOX1;

Accession NoUniprot:Q9UNE7

Uniprot Q9UNE7

Calculated MWPredicted band size: 35 kDa

Sdspage MWObserved band size: 35 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: STUB1 Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 6 seconds
All lanes:STUB1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 :STUB1 Rabbit mAb knockdown A549 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded rat heart tissue stained for STUB1 using 59818 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence STUB1 antibody (59818)

ICC/IF staining of STUB1 in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59818 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90.

If you have published an article using product 59818, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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