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p60 CAF1 Rabbit mAb#59846

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Product Detail

Product Namep60 CAF1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman

Specificityp60 CAF1 Antibody detects endogenous levels of total p60 CAF1

Immunogen DescA synthesized peptide derived from human p60 CAF1

Other NamesCAF 1A; CAF IP60; CAF-1; CAF1; CAF1A; CAF1P60; CHAF1B; MPHOSPH7; MPP7;

Accession NoUniprot:Q13112

Uniprot Q13112

Calculated MWPredicted band size: 61 kDa

Sdspage MWObserved band size: 70 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: p60 CAF1 Rabbit mAb at 1/1k dilution

Lane 1 : Caco2 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 70 kDa

Exposure time: 7 seconds
All lanes:p60 CAF1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :p60 CAF1 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human ovarian cancer tissue stained for p60 CAF1 using 59846 at 1/100 dilution in immunohistochemical analysis.
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro.

If you have published an article using product 59846, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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