Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Recombinant Rabbit Monoclonal Antibodies > CPS1 Rabbit mAb

CPS1 Rabbit mAb#59876

Availability: Yes
Total Amount:$
Product Detail

Product NameCPS1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityCPS1 Antibody detects endogenous levels of total CPS1

Immunogen DescA synthesized peptide derived from human CPS1

Other NamesCps1; CPSase 1; CPSase I; CPSASE1; MS738;

Accession NoUniprot:P31327

Uniprot P31327

Calculated MWPredicted band size: 165 kDa

Sdspage MWObserved band size: 150 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: CPS1 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : MCF-7 whole cell lysates
Lane 3 : Mouse liver lysates
Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 165 kDa
Observed band size: 150 kDa

Exposure time: 9 seconds
All lanes:CPS1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :CPS1 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for CPS1 using 59876 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CPS1 antibody (59876)

ICC/IF staining of CPS1 in HepG2 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59876 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

If you have published an article using product 59876, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;