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Syntenin Rabbit mAb#59890

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Product Detail

Product NameSyntenin Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman

SpecificitySyntenin Antibody detects endogenous levels of total Syntenin

Immunogen DescA synthesized peptide derived from human Syntenin

Other NamesMDA9; SDCBP; ST1; SYCL; Syntenin 1; TACIP18;

Accession NoUniprot:O00560

Uniprot O00560

Calculated MWPredicted band size: 32 kDa

Sdspage MWObserved band size: 32 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Syntenin Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Exposure time: 5 seconds
All lanes:Syntenin Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 :Syntenin Rabbit mAb knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Syntenin using 59890 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Syntenin antibody (59890)

ICC/IF staining of Syntenin in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59890 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA).

If you have published an article using product 59890, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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