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Location： Home > Recombinant Rabbit Monoclonal Antibodies > CBS Rabbit mAb

CBS Rabbit mAb#59902

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Product Detail

Product NameCBS Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman

SpecificityCBS Antibody detects endogenous levels of total CBS

Immunogen DescA synthesized peptide derived from human CBS

Other NamesHIP4; CBS;

Accession NoUniprot:P35520

Uniprot P35520

Calculated MWPredicted band size: 61 kDa

Sdspage MWObserved band size: 61 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: CBS Rabbit mAb at 1/1k dilution

Lane 1 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 2 seconds
All lanes:CBS Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :CBS Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human pancreatic tissue stained for CBS using 59902 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CBS antibody (59902)

ICC/IF staining of CBS in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59902 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

If you have published an article using product 59902, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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