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GNB2 Rabbit mAb#59959

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Product Detail

Product NameGNB2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityGNB2 Antibody detects endogenous levels of total GNB2

Immunogen DescA synthesized peptide derived from human GNB2

Other NamesGnb2; Gnb2l1; RACK1; Transducin beta chain 2;

Accession NoUniprot:P62879

Uniprot P62879

Calculated MWPredicted band size: 37 kDa

Sdspage MWObserved band size: 37 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: GNB2 Rabbit mAb at 1/1k dilution

Lane 1 : MCF-7 whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 6 seconds
All lanes:GNB2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :GNB2 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human cholangiocarcinoma tissue stained for GNB2 using 59959 at 1/100 dilution in immunohistochemical analysis.
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

If you have published an article using product 59959, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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