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Location： Home > Recombinant Rabbit Monoclonal Antibodies > CLSTN1 Rabbit mAb

CLSTN1 Rabbit mAb#59965

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Product Detail

Product NameCLSTN1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse

SpecificityCLSTN1 Antibody detects endogenous levels of total CLSTN1

Immunogen DescA synthesized peptide derived from human CLSTN1

Other NamesAlcadein alpha 1; alcalpha1; alcalpha2; Calsyntenin 1; CDHR12; Clstn1; CS1; CSTN1; PIK3CD; XB31alpha;

Accession NoUniprot:O94985

Uniprot O94985

Calculated MWPredicted band size: 110 kDa

Sdspage MWObserved band size: 150 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: CLSTN1 Rabbit mAb at 1/1k dilution

Lane 1 : 293T whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 110 kDa
Observed band size: 150 kDa

Exposure time: 12 seconds
All lanes:CLSTN1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :CLSTN1 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for CLSTN1 using 59965 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CLSTN1 antibody (59965)

ICC/IF staining of CLSTN1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59965 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation.

If you have published an article using product 59965, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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