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Recombinant Rabbit Monoclonal Antibodies > OXGR1 / GPR99 Rabbit mAb
OXGR1 / GPR99 Rabbit mAb#60143
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All lanes : OXGR1 Rabbit mAb at 1/1k dilution Lane 1 : Hela whole cell lysates Lane 2 : A549 whole cell lysates Lane 3 : HUVEC whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds
All lanes : OXGR1 Rabbit mAb at 1/1k dilution Lane 1 : Mouse Kidney lysates Lane 2 : Rat Spleen lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 45 seconds
All lanes : OXGR1 Rabbit mAb at 1/1k dilution Lane 1 : Wild-type Hela cell lysate Lane 2 : OXGR1 knockdown Hela cell lysate Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence OXGR1 antibody (60143) 60143 staining OXGR1 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 60143 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 60143 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
