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Location： Home > Recombinant Rabbit Monoclonal Antibodies > CYFIP1 Rabbit mAb

CYFIP1 Rabbit mAb#60146

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Product Detail

Product NameCYFIP1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityCYFIP1 Antibody detects endogenous levels of total CYFIP1

Immunogen DescA synthesized peptide derived from human CYFIP1

Other NamesCYFIP1; P140SRA1; SHYC; SRA1;

Accession NoUniprot:Q7L576

Uniprot Q7L576

Calculated MWPredicted band size: 145 kDa

Sdspage MWObserved band size: 130 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes: CYFIP1 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : Mouse liver lysates
Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 145 kDa
Observed band size: 130 kDa

Exposure time: 7 seconds
All lanes:CYFIP1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 :CYFIP1 Rabbit mAb knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity).

If you have published an article using product 60146, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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