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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ACADS / SCAD Rabbit mAb

ACADS / SCAD Rabbit mAb#60147

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Product Detail

Product NameACADS / SCAD Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityACADS / SCAD Antibody detects endogenous levels of total ACADS / SCAD

Immunogen DescA synthesized peptide derived from human ACADS / SCAD

Other NamesACAD3; ACADS; Bcd1; SCAD;

Accession NoUniprot:P16219

Uniprot P16219

Calculated MWPredicted band size: 44 kDa

Sdspage MWObserved band size: 40 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: ACADS / SCAD Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 40 kDa

Exposure time: 5 seconds
All lanes: ACADS / SCAD Rabbit mAb at 1/1k dilution

Lane 1 : Mouse heart lysates
Lane 2 : Mouse liver lysates
Lane 3 : Rat heart lysates
Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 40 kDa

Exposure time: 8 seconds
All lanes:ACADS / SCAD Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 :ACADS / SCAD Rabbit mAb knockdown A549 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for ACADS / SCAD using 60147 at 1/100 dilution in immunohistochemical analysis.
Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity).

If you have published an article using product 60147, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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