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Phospho Antibodies > PKR (Phospho-Thr451) Antibody
PKR (Phospho-Thr451) Antibody#60285
Yes
All lanes: PKR (Phospho-T451) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100ng/ml IFNa for 5min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa
Exposure time: 7 seconds
All lanes:PKR (Phospho-Thr451) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : PKR (Phospho-Thr451) Rabbit mAb knockout Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human brain tissue stained for Phospho-PKR (T451) using 60285 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Phospho-PKR (T451) antibody (60285) ICC/IF staining of Phospho-PKR (T451) in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 60285 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
