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Location： Home > Phospho Antibodies > eIF4EBP1/2/3 (Phospho-Thr32/T46) Antibody

eIF4EBP1/2/3 (Phospho-Thr32/T46) Antibody#60288

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Product Detail

Product NameeIF4EBP1/2/3 (Phospho-Thr32/T46) Antibody

Clone No.SR4378

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB,IHC

Species ReactivityHuman,Mouse

ConjugateUnconjugated

Uniprot Q13541/Q13542/O60516,,

Calculated MWPredicted band size: 13-19 kDa

Sdspage MW13-19 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : eIF4EBP1/2/3 (Phospho-Thr32/T46) Rabbit mAb at 1/1k dilution
Lane 1 : 293 whole cell lysates
Lane 2 : 293 treated with 10% FBS for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13-19 kDa
Observed band size: 13-19 kDa
Exposure time: 6 seconds
All lanes : eIF4EBP1/2/3 (Phospho-Thr32/T46) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : eIF4EBP1/2/3 (T46+T46+T32) knockout Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast tissue stained for eIF4EBP1/2/3 (Phospho-T46/T46/T32) using 60288 at 1/100 dilution in immunohistochemical analysis.

 eIF4EBP1 is a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs.

If you have published an article using product 60288, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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