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Location： Home > Recombinant Rabbit Monoclonal Antibodies > N6-methyladenosine(m6A) Rabbit mAb

N6-methyladenosine(m6A) Rabbit mAb#62258

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Product Detail

Product NameN6-methyladenosine(m6A) Rabbit mAb

Clone No.SR4572

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeIgG

PurificationProtein A

ApplicationsIP,Dot blot

Species ReactivitySpecies independent

SpecificityEndogenous

Other Namesm6A antibody N6-methyladenosine antibody

FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
DB 1:500-1:2000
The membrane was blotted with N6-methyladenosine(m6A) Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
Lane 1: N6-methyladenosine
Lane 2: Goat anti-Rabbit IgG
Lane 3: A oligo
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)

If you have published an article using product 62258, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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