Product Detail
Product NameTLR4 Mouse mAb
Clone No.MR4526
Host SpeciesMouse
ClonalityMonoclonal
IsotypeIgG1,Kappa
PurificationRecombinant Expression and Affinity purified
ApplicationsELISA,IHC,IF,FC
Species ReactivityHuman
SpecificityThis recombinant monoclonal antibody can detects endogenous levels of CD284 protein.
Immunogen DescPurified recombinant human CD284
Target NameTLR4
Other NamesTLR4 ;Toll-like receptor 4 ;hToll ;CD antigen CD284
Uniprot
O00206
Gene ID
7099
Calculated MW96kD
Sdspage MW95kDa
FormulationPhosphate-buffered solution
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Application Details
ELISA 1:5000-100000 Flow Cyt 1-2μg/Test
Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways (PubMed:10835634, PubMed:15809303, PubMed:16622205, PubMed:17292937, PubMed:17478729, PubMed:20037584, PubMed:20711192, PubMed:23880187, PubMed:27022195, PubMed:29038465, PubMed:17803912).
At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:27022195).
Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+ (PubMed:20711192).
Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:10835634, PubMed:21393102, PubMed:27022195, PubMed:36945827, PubMed:9237759).
Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production (PubMed:14517278).
In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade (PubMed:32894580).
In complex with TLR6, promotes inflammation in monocytes/macrophages by associating with TLR6 and the receptor CD86 (PubMed:23880187).
Upon ligand binding, such as oxLDL or amyloid-beta 42, the TLR4:TLR6 complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway (PubMed:23880187).
In myeloid dendritic cells, vesicular stomatitis virus glycoprotein G but not LPS promotes the activation of IRF7, leading to type I IFN production in a CD14-dependent manner (PubMed:15265881, PubMed:23880187).
Required for the migration-promoting effects of ZG16B/PAUF on pancreatic cancer cells
If you have published an article using product 62260, please notify us so that we can cite your literature.
Yes
