All lanes : HSP60 Mouse mAb-BSA and Azide free at 1/1k dilution
Lane 1 : T47D whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : A549 whole cell lysates
Lane 5 : Jurkat whole cell lysates
Lane 6 : HEK293 whole cell lysates
Lane 7 : NIH/3T3 whole cell lysates
Lane 8 : PC-12 whole cell lysates
Lane 9 : Cos7 whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa
Exposure time: 5 seconds

Formalin-fixed, paraffin-embedded human lung cancer tissues (left) and kidney cancer tissues (right) stained for HSP60 using 62261 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence HSP60 antibody (62261)

ICC/IF staining of HSP60 in 3T3-L1 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 62261 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti mouse, used at a dilution of 1/500.
Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Nuclei were counterstained with DRAQ5 fluorescent DNA dye.

Flow Cytometry Calreticulin antibody (62261)
Flow cytometric analysis of Hela cells with HSP60 Mouse mAb at 1/50 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody，purple).

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)