Product Detail
Product NameAIF Antibody FITC Conjugated
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationPurified by Protein A.
Applications ICC IF
Species ReactivityHu Ms Rt
Immunogen DescKLH conjugated synthetic peptide aa 130-180 613 derived from human AIF
Target NameAIF
ConjugateFITC
Excitation Emission494nm 518nm
Other NamesAIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6; Apoptosis-inducing factor 1, mitochondrial; Programmed cell death protein 8; AIFM1
Concentration1mg ml
Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.
Application Details
ICC=1:50-200 IF=1:50-200
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
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15 business days.
