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APAF1 Antibody FITC Conjugated#C00043F

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Product Detail

Product NameAPAF1 Antibody FITC Conjugated

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationPurified by Protein A.

ApplicationsFlow-Cyt IF

Species ReactivityHu Ms Rt

Immunogen DescKLH conjugated synthetic peptide aa 3-44 1248 derived from human Apaf-1

Target NameAPAF1NT

ConjugateFITC

Excitation Emission494nm 518nm

Other NamesCED4; APAF-1; Apoptotic protease-activating factor 1; APAF1; KIAA0413

Accession NoSwiss-Prot#O14727
NCBI Gene ID317

Uniprot O14727

Gene ID 317;

Concentration1mg ml

Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
Flow-Cyt=1:50-200 IF=1:50-200
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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