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Conjugated Antibodies > Angiotensin II type 1A receptor Antibody
Angiotensin II type 1A receptor Antibody#C03965
Yes
Lane 1: Liver (Mouse) Lysate at 40 ug;
Lane 2: Heart (Mouse) Lysate at 40 ug;
Lane 3: Placenta (Mouse) Lysate at 40 ug;
Lane 4: Kidney (Mouse) Lysate at 40 ug;
Lane 5: Embryo (Mouse) Lysate at 40 ug;
Lane 6: Cerebrum (Mouse) Lysate at 40 ug;
Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 ug;
Lane 8: Liver (Rat) Lysate at 40 ug;
Lane 9: Heart (Rat) Lysate at 40 ug;
Lane 10: Kidney (Rat) Lysate at 40 ug;
Lane 11: Cerebrum (Rat) Lysate at 40 ug;
Lane 12: Embryo (Rat) Lysate at 40 ug;
Primary: Angiotensin II type 1A receptor at 1/1000 dilution;
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution;
Predicted band size: 43 kD;
Observed band size: 46 kD;
Lane 2: Heart (Mouse) Lysate at 40 ug;
Lane 3: Placenta (Mouse) Lysate at 40 ug;
Lane 4: Kidney (Mouse) Lysate at 40 ug;
Lane 5: Embryo (Mouse) Lysate at 40 ug;
Lane 6: Cerebrum (Mouse) Lysate at 40 ug;
Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 ug;
Lane 8: Liver (Rat) Lysate at 40 ug;
Lane 9: Heart (Rat) Lysate at 40 ug;
Lane 10: Kidney (Rat) Lysate at 40 ug;
Lane 11: Cerebrum (Rat) Lysate at 40 ug;
Lane 12: Embryo (Rat) Lysate at 40 ug;
Primary: Angiotensin II type 1A receptor at 1/1000 dilution;
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution;
Predicted band size: 43 kD;
Observed band size: 46 kD;
A549(Human) Cell Lysate at 40 ug;
Primary: Angiotensin II type 1A receptor at 1/500 dilution;
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution;
Predicted band size: 41 kD;
Observed band size: 41/51 kD
Primary: Angiotensin II type 1A receptor at 1/500 dilution;
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution;
Predicted band size: 41 kD;
Observed band size: 41/51 kD
Liver (Mouse) Lysate at 40 ug;
Lung (Mouse) Lysate at 40 ug;
Adrenal gland (Rat) Lysate at 40 ug;
Primary: Angiotensin II type 1A receptor at 1/1000 dilution;
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution;
Predicted band size: 41 kD;
Observed band size: 56 kD
Lung (Mouse) Lysate at 40 ug;
Adrenal gland (Rat) Lysate at 40 ug;
Primary: Angiotensin II type 1A receptor at 1/1000 dilution;
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution;
Predicted band size: 41 kD;
Observed band size: 56 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, DAB staining.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
