Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TNNI3 in samples. An antibody specific for TNNI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.