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Location： Home > Methyl / Acetyl Antibodies > Histone H3K14me2 Polyclonal Antibody

Histone H3K14me2 Polyclonal Antibody#HW035

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Product Detail

Product NameHistone H3K14me2 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IF

Species ReactivityHuman,Mouse,Rat

Immunogen TypePeptide

Immunogen DescA synthetic peptide of human DiMethyl-Histone H3-K14

Target NameHistone H3

ConjugateUnconjugated

Other NamesH3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A

Accession NoUniprot:Q16695
GeneID:8290

Uniprot Q16695

Gene ID 8290

Sdspage MW17KDa

Concentration1.0mg/ml

FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K14 antibody.
Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb.
Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb.
Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

If you have published an article using product HW035, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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