Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Methyl / Acetyl Antibodies > Histone H3(Tri-methyl-K9) Mouse Monoclonal Antibody

Histone H3(Tri-methyl-K9) Mouse Monoclonal Antibody#HW051

Datasheet PDF
Availability： Not Available
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameHistone H3(Tri-methyl-K9) Mouse Monoclonal Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

ApplicationsWB

Species ReactivityHu Rt Ms

SpecificityThe Histone H3(Tri-methyl-K9) antibody can detects endogenous Histone H3(Tri-methyl-K9) protein.

Immunogen DescA synthetic Tri-methylated peptide corresponding to residues surrounding K9 of human histone H3.

Target NameHistone H3(Tri-methyl-K9)

Accession NoSwiss-Prot#:P68431

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Sdspage MW15 kDa

Concentration1.0mg/ml

FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C

Application Details
WB 1:1000-3000
Western blot analysis of Hela cells, using #HW051 diluted at 1) 1:2,000 2) 1:5,000
Western blot analysis of 1) Rat Testis Tissue, 2) Raw264.7, using #HW051 diluted at 1:2000.
1¡¢Input: Hela Cell Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #HW051 1:1,000
Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

If you have published an article using product HW051, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors