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Location： Home > Methyl / Acetyl Antibodies > Histone H3(Di-Methyl-Lys4) Rabbit Polyclonal Antibody

Histone H3(Di-Methyl-Lys4) Rabbit Polyclonal Antibody#HW068

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Product NameHistone H3(Di-Methyl-Lys4) Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

Species ReactivityHu Rt Ms

SpecificityThe Histone H3(Di-methyl-Lys4) Rabbit Polyclonal Antibody detects endogenous Histone H3 (Di-methyl-Lys4) protein.

Immunogen Typepeptide

Immunogen DescA synthetic Di-methylated peptide corresponding to residues surrounding Lys4 of human histone H3.

Target NameHistone H3(Di-Methyl-Lys4)

ConjugateUnconjugated

Other NamesH3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody

Accession NoSwiss-Prot#:P68431

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Sdspage MW17kd

Concentration1.0mg/ml

FormulationPurified Rabbit IgG, 1mg/ml in PBS pH7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:1000~1:2000
Western blot analysis of 1) Hela, 2)3T3 , 3) Raw264.7, using #HW068 diluted at 1:2,000.
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

If you have published an article using product HW068, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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