Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Methyl / Acetyl Antibodies > Histone H3(Acetyl-Lys27) Rabbit Polyclonal Antibody

Histone H3(Acetyl-Lys27) Rabbit Polyclonal Antibody#HW077

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameHistone H3(Acetyl-Lys27) Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAffinity purification using immunogen.

ApplicationsWB, IHC, IF, IP, ELISA

Species ReactivityHuman, Mouse, Rat, Monkey, Arabidopsis thaliana

SpecificityThe Histone H3(Acetyl Lys27) Rabbit Polyclonal Antibody detects endogenous Histone H3 (Acetyl Lys27) protein.

Immunogen DescA synthetic acetylated peptide corresponding to residues surrounding Lys27 of human histone H3.

Target NameHistone H3(Acetyl-Lys27)

ConjugateUnconjugated

Other NamesH3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody

Accession NoSwiss-Prot#:P68431

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Sdspage MW17kd

Concentration1.0mg/ml

FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C

Application Details

 WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; IP 1:200; ELISA 1:5000

Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using #HW077 diluted at 1:1,000.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys27) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Hela cell. Histone H3 (Acetyl Lys27) Polyclonal Antibody(red) was diluted at 1:200(4° overnight).

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

If you have published an article using product HW077, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors