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Location： Home > Secondary Antibodies > AffiniPure Goat Anti-Rabbit IgG (H+L)

AffiniPure Goat Anti-Rabbit IgG (H+L)#L3001

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Product NameAffiniPure Goat Anti-Rabbit IgG (H+L)

Host SpeciesGoat

ClonalityPolyclonal

IsotypeWhole IgG

PurificationThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

SpecificityIgG (H+L)

ConjugateUnconjugated

StorageStore at 2-8°C under sterile conditions

Target SpeciesRabbit

Application Details

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Preservative: None 

Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use. 

 

Physical State: Sterile-filtered liquid 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species.

If you have published an article using product L3001, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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